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While singly-housed animals may experience stress that impacts metabolism, body weight, and adiposity 65,66,67,68,69 , the singly-housed animals in this study were treated identically (to the best of the experimenter’s ability) to minimize and equalize stress across all animals and reduce confounding variables

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A 250 mcg dose requires drawing 0.125 mL (12.5 units on a U-100 insulin syringe)